The invention relates to a maca composition for relieving the climacteric symptoms of females and a preparation method thereof. The maca composition is prepared from maca extract, prepared rehmannia root extract, radix ophiopogonis extract, spina date seed extract, poria cocos extract and extract from pueraria. Natural plants are adopted as the raw materials of the maca composition, maca and traditional Chinese medicine are compounded, active ingredients are extracted in combination of the modern science and technology, the traditional Chinese medicine theory is followed in raw material composition, and on the one hand, the estrogen level in the bodies of the menopausal females is adjusted by nourishing the ovary and nourishing kidney yin; on the other hand, the sub-health status of the menopausal females is relieved by relieving pressure, tranquilizing and allaying excitement and boosting immunity, and therefore the effect of relieving the climacteric syndromes of the females is achieved. It is proved through animal experiments that the maca composition has remarkable effects on relieving the climacteric syndromes of the females.