The invention relates to a three-dimensional reading device of a radio frequency label. The device comprises a flat board antenna and a radio frequency reader writer, wherein the flat board antenna is distributed with multiple radio frequency antennas used for emitting radio frequency signals, the radio frequency antennas are parallelly and sequentially distributed from down to up in a staggered mode, the radio frequency reader writer comprises a switching control module used for switching and conducting the radio frequency antennas, a radio frequency module used for establishing connection with the radio frequency antennas after conduction of the radio frequency antennas and sending the radio frequency signals to the radio frequency antennas, switching and control signals generated by the radio frequency reader writer are received by the switching control module and identified, and the corresponding radio frequency antennas are switched and conducted. The radio frequency module is used for establishing connection with the conducted radio frequency antennas and sending the radio frequency signals generated by the radio frequency reader writer to the radio frequency antennas, and the radio frequency antennas are used for sending out the radio frequency signals. Through the device, reading missing can be effectively prevented, and the reading success rate is improved.