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CN105378057A 用于丁醇生产的甘油3-磷酸脱氢酶 失效 - 放弃专利权

用于丁醇生产的甘油3-磷酸脱氢酶
摘要：
本文提供了对NADH具有增大的KM的甘油-3-磷酸脱氢酶(GPD)以及对NADH和NADPH具有基本上相同的亲和力和/或受到甘油-3-磷酸的反馈抑制的GPD酶。本文还提供了包含编码GPD的异源基因和编码GPD的内源基因中的缺失或破坏的重组微生物。还提供了包含编码GPD的异源基因和丁醇生物合成途径的重组微生物。还提供了生产丁醇的方法，所述方法包括提供本文所述的重组微生物以及使所述重组微生物与至少一种可发酵碳底物在其中产生丁醇的条件下接触。
摘要（英）：

Provided herein are glycerol-3 -phosphate dehydrogenase (GPD) enzymes with increased KM for NADH and GPD enzymes with substantially the same affinity for NADH and NADPH and/or are feedback inhibited by glycerol-3-phosphate. Also provided herein are recombinant microorganisms comprising a heterologous gene encoding GPD and a deletion or disruption in an endogenous gene encoding GPD. Also provided are recombinant microorganisms comprising a heterologous gene encoding GPD and a butanol biosynthetic pathway. Further provided are methods of producing butanol comprising providing the recombinant microorganisms described herein and contacting the recombinant microorganism with at least one fermentable carbon substrate under conditions wherein butanol is produced.

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