The invention relates to a protective film capable of preventing blue light and blocking ultraviolet light. The protective film is formed by a wearing layer (1), a first polyester film (2-1), a blue light-preventing transparent adhesive layer (3), a second polyester film (2-2), an ultraviolet blocking pressure-sensitive adhesive layer (4) and a release film (5), and all the layers are combined into a whole; the blue light-preventing transparent adhesive layer (3) is a mixed material formed by a compound light stabilizer, an adhesive, a curing agent and a solvent, is used for coating the other surface of the first polyester film (2-1), is placed at the temperature of 90 to 150 DEG C for 40 to 120s, and then is cured to obtain the blue light-preventing transparent adhesive layer (3), wherein the compound light stabilizer is prepared by mixing a nanocrystalline metal oxide particle light stabilizer and a triazine derivative light stabilizer, and the adhesive is an acrylate adhesive or a polyurethane adhesive. The protective film provided by the invention can effectively protect human eyes from being harmed by blue light, and meanwhile, has the functions of blocking the ultraviolet light, protecting a product or a glass surface from being scratched, and the like.