The invention provides a high-purity cardiac muscle cell primary culture method and is suitable for the field of biotechnology and medical science. The high-purity cardiac muscle cell primary culture method particularly comprises the steps that an isolated heart of a suckling mouse is sheared into tissue fragments; digestion is conducted on the tissue fragments through improved prepared enzyme digestive juice for multiple times, cardiac muscle cell suspension is prepared, resuspension is conducted, and then cardiac muscle cells are inoculated in a culture dish A for differential-time wall sticking; cardiac muscle cell suspension in the culture dish A is sucked into a culture dish B, cardiac muscle cells which sink to the bottom of the culture dish A and do not stick to the wall in the culture dish A are cleaned and resuspended, and then the resuspended cardiac muscle cells are transferred into the culture dish B to be cultured. Compared with the prior art, the high-purity cardiac muscle cell primary culture method has the advantages that the method is simple and effective, the operability is strong, the number of obtained cardiac muscle cells is large, the purity is high, the wall sticking rate is good, and the survival rate is high.