The invention relates to application of LCE3E to diagnosis and treatment of an oral cancer. According to the application, transcriptome deep sequencing analysis is performed through a high-throughput sequencing platform to preliminarily screen out LCE3E genes obvious in differential expression in oral squamous cell carcinoma tissue and normal tissue, a further RT-PCR experiment proves high expression of the LCE3E genes in the oral squamous cell carcinoma tissue, and an siRNA interference experiment shows that interference to expression of the LCE3E genes can effectively suppress proliferation of oral cancer cells. Oral cancer molecular markers in the application have significant clinical practical application value.