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发明公开
CN105400775A 一种无损伤提取中华鲟DNA的方法 有权

一种无损伤提取中华鲟DNA的方法
摘要：
本发明涉及生物工程领域，特别涉及一种无损伤提取中华鲟DNA的方法。所述方法包括获取样本、样本的处理、裂解细胞、沉淀蛋白质、沉淀DNA、洗涤DNA、获取DNA。本发明建立了一套完整的提取中华鲟粘液的方法，本发明可以快速有效的提取中华鲟DNA，提取的DNA条带清晰，无拖尾现象，说明提取的DNA质量高，不收蛋白质的污染，结果稳定可靠。
摘要（英）：

The invention relates to the field of bioengineering, in particular to a method for harmlessly extracting Chinese sturgeon DNA. The method includes the steps of obtaining a sample, processing the sample, conducting cell lysis, precipitating proteins, precipitating DNA, washing DNA and obtaining DNA. The complete method for extracting Chinese sturgeon mucus is established, Chinese sturgeon DNA can be rapidly and effectively extracted, extracted DNA stripes are clear and free of trailing, and it is shown that extracted DNA is high in quality and free of protein contamination and stable and reliable in result.

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