The invention discloses a trace spherical charging device for centrifuge underwater explosion experiments and a manufacturing method thereof. The trace spherical charging device comprises a spherical shell made of a preservative film; a main charging device is arranged in the shell; a cylindrical opening is formed in the shell in an extending mode; a detonating cord connected with the main charging device is arranged in the opening; a booster explosive is arranged between the detonating cord and the main charging device; the cylindrical opening is sleeved with a heat shrink tube. The trace spherical charging device for centrifuge underwater explosion experiments has the characteristics of being complete in detonation, high in dose precision, high in charging material mechanical property and capable of meeting the requirement for the specific dose precision and mechanical environment of centrifuge underwater explosion experiments. According to the manufacturing method of the trace spherical charging device for centrifuge underwater explosion experiments, the spherical charging device is divided into two hemispherical charging device bodies which are bonded to be in a spherical shape through shellac varnish, and the preparation precision is high.