The invention discloses an isolated microgrid frequency modulation control method based on measured frequency response identification. The method provides a frequency modulation control strategy aiming at the operation features of an isolated microgrid and based on a principle of segmented frequency modulation control. According to the control strategy, the emergency degrees of the frequency modulation control are graded according to the frequency deviations of the microgrid, different active power control modes are used in different control areas; control parameters based on the measured frequency response identification are used, and thereby renewable energy sources can be maximally used for generating power, meanwhile the charging and discharging times of an energy storage battery are reduced, and an oscillation problem due to the fact that power modulation speeds are inconsistent is avoided. When disturbance occurs, the strategy can support the frequency of the microgrid, and improve the stability of the frequency of the microgrid.