The embodiment of the invention discloses a power grid adjustment and control end anti-misoperation locking method. The method includes the following steps that: full-grid locking is performed on administered equipment under centralized monitoring; telemetering and remote signaling data from a substation to an adjustment and control end of a power grid are acquired in real time; the working condition of the substation is judged according to the telemetering and remote signaling data; corresponding administered equipment in full-grid locking is unlocked according to a remote control instruction of the adjustment and control end and the working condition of the substation; whether the unlocking of the corresponding administered equipment is successful or not is judged; if the unlocking is successful, anti-error verification is performed on the remote control instruction, and whether the remote control instruction accords with a preset anti-error verification condition is judged; and if the remote control instruction accords with the preset anti-error verification condition, the remote control instruction is executed. According to the power grid adjustment and control end anti-misoperation locking method, only when the unlocking of the equipment is successful, and the anti-error verification on the remote control instruction is successful, the remote control instruction can be executed, and corresponding equipment can operate under control, and therefore, the adjustment and control end of the power grid can realize remote switching operation of an unattended substation and overall anti-misoperation locking, and the security protection ability of the substation can be improved.