The invention relates to a vaginal speculum applied to photodynamic therapy. The vaginal speculum applied to photodynamic therapy comprises an inserting part suitable for entering into vagina, a protruding part applicable to at least a near end and left outside the vagina, and a handle part, wherein the inserting part is provided with a first wall closed circumferentially and made of a transparent material, and the first wall defines a passage extending from the near end to the far end of the inserting part; the protruding part is provided with a second wall extending circumferentially and connected to the near end of the inserting part, and the second wall defines a through hole extending from the near end to the far end of the protruding part and communicated with the passage of the first wall; and the handle part is connected to the near end of the protruding part and avoids the passage. The vaginal speculum provided by the invention can avoid inconvenience caused by that human tissue is caught in the vaginal speculum, damage to vaginal wall caused by scraping cannot be caused, and the operation is easy.