The invention discloses a three-color nano-rubber EVA (Thylene-Vinyl Acetate) injection die. The three-color nano-rubber EVA injection die comprises an upper die assembly and a bottom die assembly, wherein the upper die assembly comprises an upper die separating plate and an upper die which are matched with each other; the upper die and the upper die separating plate can be combined, and an upper die core for forming a third-color finished product is arranged in the combination; the bottom die assembly comprises a bottom die separating plate and a bottom die which are matched with each other; the bottom die and the bottom die separating plate are combined, and a bottom die core for forming a first-color finished product and a second-color finished product is arranged in the combination; injection ports for pouring the nano-rubber EVA are formed in the side edges of the same sides of the upper die assembly and the bottom die assembly; a third-color injection port is formed in the upper die assembly; a first-color injection port and a second-color injection port are formed in the bottom die assembly; the first-color injection port, the second-color injection port and the third-color injection port correspondingly communicate with a first-color material channel, a second-color material channel and a third-color material channel which extend to the upper die core and the bottom die core.