A methacrylate fluorescent monomer with thiazolepyridine structure and a preparation method thereof are disclosed. The methacrylate fluorescent monomer with the thiazolepyridine structure is prepared using thiazolepyridine-3-carboxyl and hydroxyethyl methacrylate as starting materials or thiazolepyridine-3,7-dicarboxyl and hydroxyethyl methacrylate as starting materials. A synthetic method of the invention has few side products, the prepared methacrylate fluorescent monomer is high in product purity and high in quantum yield, the synthetic method is simple to operate, has mild reaction conditions and allows large-scale preparation and easy industrialization, and fluorescent polymers are easy to prepare through polymerization reaction.