The invention discloses a synthetic method and application of a carbofuran amination hapten, and relates to a biological chemical technology. The synthetic method of the novel carbofuran amination hapten is simple and effective in synthetic step and high in yield, and avoids the harmfulness of a lot of phosgene and methylbenzene used in a conventional triphosgene synthetic method in preparation of carbofuran carboxylation hapten. According to the synthetic method, furan phenol is used as an initial raw material, and by virtue of esterification, aminolysis, Boc protection and de-protection reaction, an arm chain containing 3 carbons and amino terminals is introduced to form an amination hapten; and a glutaraldehyde method is adopted to ensure that the hapten is subjected to condensation with amino groups on a carrier protein to expose a carbofuran antigenic determinant to the maximum extent. An immune animal experiment shows that the artificial antigen prepared by the method disclosed by the invention has good immunogenicity. The hapten and the artificial antigen provided by the invention can be completely applied to carbofuran immunoassay to meet domestic requirements for detecting carbofuran residues, and have a broad application prospect.