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发明授权
CN105441445B miR528的调控位点及其应用 失效 - 权利终止

miR528的调控位点及其应用
摘要：
本发明涉及水稻OsmiR528编码基因MIR528及OsmiR528调控位点(Os06g06050、Os06g37150、Os08g04310、Os08g42640和Os07g38290)在控制水稻生育期和病毒抗性方面的作用。本发明人通过OsmiR528过量表达和敲除水稻株系的研究，发现OsmiR528能够影响水稻抽穗期和抗水稻条纹叶枯病毒(RSV)能力：即过量表达OsmiR528会导致抽穗期提前和对RSV病毒敏感，而敲除OsmiR528则会导致抽穗期延迟和抗RSV病毒。而且MIR528在水稻不同品种中存在直接影响OsmiR528表达水平的调控序列多态性，以上结果可应用于水稻杂交育种及分子辅助育种。
摘要（英）：

Provided in the present invention is the use of regulatory sites (Os06g06050, Os06g37150, Os08g04310, Os08g42640 and Os07g38290) of rice miR528 encoding genes MIR528 and miR528 in the control of rice growing period and viral resistance. By the study of miR528 overexpression and knockout strains of rice, we found miR528 can affect rice heading period and anti Rice Stripe Virus (RSV) capability, and the overexpression of miR528 can result in an earlier heading period and sensitivity to RSV virus, while the knockout of miR528 will result in a delayed heading period and resistance to RSV virus. The presence of MIR528 in different rice varieties directly affects the expression level regulatory sequence polymorphism of miR528 and can be used for rice hybrid breeding and molecular assisted breeding.

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