利索-全球专利检索

  1. 登录
  2. 注册
发明公开
CN105441473A 一种谷氨酸生产菌株及其制备方法 无效 - 驳回

一种谷氨酸生产菌株及其制备方法
摘要：
本发明涉及一种谷氨酸生产菌株的制备方法，该方法包括将生产谷氨酸的野生菌株中编码谷氨酸代谢通路上的酶的基因进行密码子偏好性改造，密码子偏好性改造的方式为：通过将促进谷氨酸产生的基因所对应的密码子至少部分替换为偏好性高的密码子，和/或通过将抑制谷氨酸产生的基因所对应的密码子至少部分替换为偏好性低的密码子，得到经密码子偏好性改造的基因序列。本发明的方法能够在不改变基因组拷贝数和谷氨酸蛋白序列的情况下，提高谷氨酸的产量，制得产生谷氨酸能力较强的谷氨酸生产菌株。
摘要（英）：

The present invention relates to a preparation method of a glutamic acid producing strain, the method comprises codon preference transformation of a gene for encoding an enzyme on a glutamic acid metabolic pathway in a glutamic acid producing wild strain, the manner of the codon preference transformation is as follows: a codon corresponding to a gene for promoting glutamic acid producing is at least partially replaced with a high preference codon, and/or a codon corresponding to a gene for inhibiting the glutamic acid producing is at least partially replaced with a low preference codon to obtain a gene sequence with the transformed codon preference. The method can improve the yield of glutamic acid without changing a genomic copy number and a glutamic acid protein sequence, and the glutamic acid producing strain with high glutamic acid producing capability can be obtained.

0/0