The invention discloses a buffering and isolating mainframe bracket which comprises a bottom plate and a locating component, wherein side plates are arranged on the two sides of the bottom plate; universal wheels are arranged at the four bottom corners of the bottom plate; the locating component comprises locating rods and elastic parts; each locating rod comprises a horizontal rod part and a brake rod part which are connected; the horizontal rod parts are hinged to the bottom plate, and are matched with the bottom plate through the elastic parts; a buffer plate and a baffle plate inserting groove are arranged on the inner side wall of each of the two side plates; each buffer plate is connected with the corresponding side plate through buffer springs; and the baffle plate inserting grooves are positioned in the front sides of the locating rods. When a computer mainframe box is moved in place, a user only needs to push the mainframe box to press the locating rods, the brake rod parts of the locating rods are propped against the ground, and the fixing is achieved; and when the mainframe box needs to be moved, the computer mainframe is pushed away from the horizontal rod parts of the locating rods, the locating rods automatically tilt under the action of the elastic parts, the integral computer mainframe box can be moved, baffle plates can be externally connected on the baffle plate inserting grooves for blocking the mainframe box, and the buffer plates can prevent the collision and friction of the mainframe box and the side wall.