The embodiment of the invention provides a manual negative pressure treatment device. The manual negative pressure treatment device comprises a manual negative pressure source, an air inlet check valve, an exhaust check valve and a liquid collecting device. The manual negative pressure source is used for generating negative pressure in a manual actuating mode and is provided with a two-way air circuit connector. The air inlet check valve is connected with the two-way air circuit connector through an air circuit and used for providing negative pressure for the liquid collecting device to pump and suck wound exudate of a patient. The exhaust check valve is connected with the two-way air circuit connector through an air circuit and used for discharging air in the manual negative pressure source. The liquid collecting device is connected with the air inlet check valve and dressings through air circuits respectively and used for storing the wound exudate of the patient. The manual negative pressure source comprises an injector-shaped negative pressure pump and at least one a rebounding device connected with the injector-shaped negative pressure pump and used for making the injector-shaped negative pressure pump perform one-way actuation. The manual negative pressure treatment device is easy to operate and use, physical strength is saved, and stability of the manual negative pressure treatment device in the treatment process is improved.