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发明授权
CN105463019B 一种增强hUCMSCs增殖能力的方法及其应用 有权

一种增强hUCMSCs增殖能力的方法及其应用
摘要：
本发明涉及生物技术领域，具体是snoRA7A的新用途，以及一种在体外培养条件下增强hUCMSCs增殖能力的方法。本发明成功构建snoRA7A的表达质粒，将该质粒转染hUCMSCs，获得snoRA7A高表达的hUCMSCs，较hUCMSCs具有更高的增殖能力，且多能性更好；可在体外培养条件下，获取大量功能状态较好的细胞，解决了hUCMSCs在体外培养过程中随着传代次数增加，增殖能力等生物活性降低的问题；本发明为hUCMSCs体外培养传代过程中细胞增殖能力的维持以及增强提供了新思路，为hUCMSCs的临床应用提供了更广阔的前景。
摘要（英）：

The invention relates to the technical field of biology, in particular to new application of snoRA7A and a method for enhancing the proliferative capacity of hUCMSCs under an invitro culture condition. According to the method for enhancing the proliferative capacity of the hUCMSCs, expression plasmids of the snoRA7A are successfully constructed, the plasmids are used for transfecting the hUCMSCs, hUCMSCs achieving high expression of the snoRA7A are obtained, and compared with original hUCMSCs, the obtained hUCMSCs are higher in proliferative capacity and better in pluripotency. A larger number of cells with a better functional state can be obtained under the invitro culture condition, and the problem that biological activity of the proliferative capacity and the like of the hUCMSCs is decreased along with increase of the passage number in the invitro culture process is solved. By means of the method for enhancing the proliferative capacity of the hUCMSCs, a new idea is provided for maintaining and enhancing of the cell proliferative capacity of the hUCMSCs in invitro culture and passage process, and a wider prospect is provided for clinical application of the hUCMSCs.

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