A tissue-culture rapid propagation method for arundina graminifolia is characterized by comprising step 1, selecting and disinfecting explant materials, concretely selecting a high bud as an explant, and performing disinfection for usage; step 2, performing inducing culture to obtain adventitious bud, concretely, cutting the top of the high bud, inoculating an adventitious bud culture medium with the high bud, so as to obtain the adventitious bud through inducing; step 3, performing propagation culture to clustered buds, concretely, inoculating a clustered-bud induction medium with the adventitious bud, so as to obtain the clustered buds, and then inoculating a propagation medium with the clustered buds, so as to obtain a clustered-bud block mass; step 4, performing seedling strengthening and rooting culture, concretely, cutting the large clustered-bud block mass obtained in the step 3 into small block masses, then inoculating the seedling-strengthening rooting medium with the small clustered-bud block masses obtained in the step 3, wherein the small clustered-bud block mass grow vigorously and new roots are grown; and step 5, performing seedling hardening and domestication transplanting, when a seedling grows to 4-8 cm and has 1-3 roots, performing seedling-hardening culture, then taking the seedling out, immersing in a thiophanate methyl solution, and finally transplanting by using moss. The variation probability of arundina graminifolia during subculture proliferation is reduced.