The invention discloses a preparation method of a conductive silver film, and belongs to the technical field of two-dimensional silver nanomaterial self-assembling. The method includes: firstly, solvent in dispersing liquid of silver nanoparticles wrapped by surface active agents on the surface is removed by employing the rotary evaporation method, and dry silver nanoparticles wrapped by the surface active agents on the surface are obtained; the dry silver nanoparticles wrapped by the surface active agents on the surface are dispersed in polar solvent, a substrate after hydrophobic treatment is immersed into the polar solvent, and the silver nanoparticles are self-assembled on the substrate to from a silver film; and the substrate is taken out, and sealed and soaked in treating agents after complete volatilization of the polar solvent, and the conducive silver film is finally obtained. According to the synthesized silver conductive silver film, the porosity is low, the conductivity is excellent, the film-forming property is good, the production cost is low, the conversion rate is high, the operation of the preparation process is simple, industrialization and mass production can be realized, and problems of large pores and the requirement of high-temperature sintering of the conductive silver film in the prior art are solved.