The present invention provides a composition, kit, and method for detecting hypoxia or diagnosing hypoxia-related diseases, the composition containing a material for detecting arachidonic acid and a derivative thereof. The composition, kit, and method according to the present invention can conveniently and promptly detect hypoxia through the detection of a biomarker in a biological sample, and thus can be useful in the prevention or early diagnose of diseases caused by hypoxia, the determination of the severity of diseases and therapeutic effects, tracking of diseases, or the like.