The invention relates to a living cell product preservation solution and preparation and using methods thereof. The living cell product preservation solution is prepared from catechinic acid, glucose, human serum albumin, sodium bicarbonate and preservation base fluid. The preservation base fluid is composed of a 0.9% sodium chloride solution and a compound electrolyte solution. The catechinic acid, the glucose, the human serum albumin and the sodium bicarbonate are added into the preservation base fluid, a 0.22-micrometer filter film is used for filtration sterilization, and the living cell product preservation solution is obtained. Living cell product preservation solution resuspension cells are used, the cell concentration is 0.5*10<7>-5*10<7> cells/ml, and normal temperature preservation is carried out for 0-72 h. The living cell product preservation solution can be used for preserving living cells for a long time, and living cells still have high activity and motility rate after being preserved through the preservation solution for 72 h. The living cell product preservation solution can preserve living cells at normal temperature.