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发明公开
CN105505850A 一种煤泥水中残留聚丙烯酰胺的生物降解方法 无效 - 驳回

一种煤泥水中残留聚丙烯酰胺的生物降解方法
摘要：
本发明涉及一种煤泥水中残留聚丙烯酰胺的生物降解方法，尤其是涉及一种利用驯化后的球红假单胞菌降解煤泥水中残留聚丙烯酰胺的方法，其特征在于：包括以下步骤。种子的制备、降解菌的驯化、单菌落的分离、菌悬液的配制、将菌悬液按1～5％的量接种到含聚丙烯酰胺的降解培养基中，在温度为25～40℃，初始pH＝5～7，搅拌速度为100～140r/min时培养5～8d，即可取得较好的降解效果。采用上述方法驯化的球红假单胞菌能以聚丙烯酰胺为唯一氮源进行降解，并取得很好的降解效果，同时不会对环境造成二次污染。
摘要（英）：

The invention relates to a method for biodegrading residual polyacrylamide in coal slurry water, particularly a method for degrading residual polyacrylamide in coal slurry water by using domesticated Rhodopseudomonas sphaeroides. The method is characterized by comprising the following steps: preparing a seed, domesticating degradation bacteria, separating single bacterial colonies, preparing a bacterium suspension, inoculating the bacterium suspension into a polyacrylamide-containing degradation culture medium according to the amount of 1-5%, and culturing at 25-40 DEG C at the stirring rate of 100-140 r/min under the initial pH value of 5-7 for 5-8 days, thereby obtaining favorable degradation effects. The Rhodopseudomonas sphaeroides domesticated by the method can be used for degradation by using the polyacrylamide as the unique nitrogen source, obtains favorable degradation effects, and can not cause secondary pollution on the environment.

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