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发明公开
CN105505924A 检测EGFR基因L858R位点的特异性引物、探针及试剂盒 无效 - 撤回

检测EGFR基因L858R位点的特异性引物、探针及试剂盒
摘要：
本发明涉及分子检测，具体公开了用于检测EGFR基因L858R突变位点的特异性引物、探针及试剂盒。所述特异性引物的核苷酸序列如SEQ ID NO 1～2所示，所述探针的核苷酸序列如SEQ ID NO 3～4所示。利用本发明提供的特异性引物和MGB-Taqman探针应用探针方法检测EGFR基因L858R，不仅适用于对肿瘤组织，石蜡包埋组织来源的核酸进行检测，更加适用于对血液循环游离核酸中EGFR基因L858R突变的位点的检测；且结合该检测体系和微滴式数字PCR系统可对核酸样本的突变频率进行准确检出。
摘要（英）：

The invention relates to molecular detection, and particularly discloses a specific primer, probe and kit for detecting an EGFR gene L858R locus. A nucleotide sequence of the specific primer is shown as SEQ ID NO. 1 to 2, and a nucleotide sequence of the probe is shown as SEQ ID NO. 3 to 4. The specific primer and the MGB-Taqman probe provided by the invention adopt a probe method to detect the EGFR gene L858R, and are not only suitable for detecting nucleic acid from a tumor tissue and a paraffin-embedded tissue, but also suitable for detecting a mutant locus of the EGFR gene L858R in free circulating nucleic acid of blood; and moreover, by combining a detection system and a micro-drop digital PCR system, the mutation frequency of a nucleic acid sample can be accurately detected.

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