The invention provides a method and apparatus for configuration of production operation. The method comprises acquiring information for multi items of production operation to be performed in a preset time quantum according to a priority sequence; according to the information for multi items of production operation, determining the initial population; and performing the following operations for every generation of population successively: calculating the completion time, required by configuration of production operation, represented by every gene in the current generation of population, and determining whether genes satisfying preset conditions exist according to the completion time; if not, selecting a preset quantity of pairing genes according to the probability that every gene is selected in the current generation of population, and performing crossover operation and mutation operation on every pair of pairing genes to acquire genes in the next generation of population; if so, acquiring the genes satisfying the preset conditions and stopping the circulation; and determining the genes satisfying the preset conditions as the optimum configuration for production operation.