The invention discloses a lily virus X (LVX), lily prunus necrotic ringspot virus (PNRSV) and lily strawberry latent ringspot virus (SLRSV) composite semi-quantitative detection colloidal gold card and a preparation method thereof; for improving the use efficiency and the field popularizing rate of a colloidal gold immunochromatographic detection method and reducing the detection cost, synchronous, rapid and semi-quantitative detection of lily viruses is achieved; the number of detection lines is increased to 9, then the detection lines are coated by antibodies with known amounts and concentration difference, the colloidal gold card which can perform simultaneous semi-quantitative detection of LVX, PNRSV and SLRSV is developed, and the requirements of lilium spp. large-scale detoxification and rapid semi-quantitative detection of the lily viruses in fields are met; the colloidal gold card has high detection efficiency, the detection cost is further reduced, and the types of viruses detected at one time are more complete; in addition, the colloidal gold card has the advantages of simple operation, high accuracy, strong specificity, and good repeatability.