The invention provides a PXI bus-based portable intelligent simulation unit, which comprises a PXI case, a network interface board, a time service unit board, an analogue collection board, a switching value input board and an operation loop board, wherein the PXI case, the network interface board and the time service unit board are connected with the analogue collection board, the switching value input board and the operation loop board through a PXI bus. The PXI bus-based portable intelligent simulation unit provided by the invention integrates three functions of a merging unit, an intelligent terminal and a time service unit; a configuration method is relatively simple and convenient; and a simple and convenient test environment is provided for testing of protection and measurement and control devices.