The invention relates to a method for making a permanent glass slide specimen of bryophyte. Gum Arabic, double distilled water, glycerin and chloral hydrate are mixed with stirring at normal temperature, and a liquid mounting medium is prepared; moss leaves or plants are placed in the double distilled water, after infiltration, the moss leaves or plants are placed in the center of a slide glass with a droplet of double distilled water, and the moss leaves or plants are observed under a 10x microscope for adjusting shapes; a piece of filter paper is used for absorbing double distilled water which is 1 millimeter or more far away from the specimen, till the circumference of the moss specimen is moistened without excessive double distilled water; a glass rod is used for dipping 1-2 droplets of the mounting medium, and the droplets are dropped on the moss specimen, a piece of cover glass is covered without bubbles contained, the specimen is placed at normal temperature till the mounting medium is dried and can fix the specimen without movement, a label is plastered on the slide glass, and the permanent glass slide specimen of bryophyte is prepared. Compared with prior art, the produced specimen has the advantages of good transparency, simple method, long preservation time limit, and good preservation effect of original state of the specimen.