The invention relates to graphene oxide modified bone cement as well as a preparation method and application thereof. The bone cement is composed of powder and liquid, wherein the powder comprises 1-50% of graphene oxide modified hydroxyapatite and 50-99% of calcium sulfate, and the main component of the liquid is graphene oxide aqueous solution or water. The graphene oxide modified bone cement provided by the invention is applied to multiple filling and repairing materials for fracture fixation and bone defect. Graphene oxide and hydroxyapatite are directly combined and modified so as to prevent impurities from entering during modification by graphene oxide in a hydroxyapatite preparation process. Hydroxyl groups in graphene oxide and hydroxyl groups in hydroxyapatite form hydrogen bonding so as to ensure that when the bone cement is operated, no dross or powder falls off or peels off, and no solid-liquid separation phenomenon exists; and by providing graphene oxide for modifying hydroxyapatite, the bone cement has favorable antibacterial property, water scattering resistance and biocompatibility. The graphene oxide modified bone cement provided by the invention is simple and convenient in process and is widely used for biological materials.