The invention provides a layering continuous well testing system and a use method thereof. The layering continuous well testing systems which are placed in a casing at interval comprise drainage test assemblies, straddle assemblies, and perforation assemblies connected from top to bottom. The cut-through cavity of the drainage test assemblies is communicated with the cut-through cavity of the straddle assemblies, to form a production channel. The straddle assemblies comprise a pressure transmitting joint, an upper packer, an upper pilot-controlled sieve tube, a lower packer, and a lower pilot-controlled sieve tube. In the production channel, pressure transmitting pipes which extend from the pressure transmitting joint and are communicated with an ignition head are arranged at interval. The pressure transmitting hole on the pressure transmitting joint is communicated with the upper port of the pressure transmitting pipe, to form a pressurization channel. The upper pilot-controlled sieve tube is provided with a first diversion channel which is separated from the production channel. The pressurization channel is injected with fluid, so the first diversion channel is communicated with the production channel. The lower pilot-controlled sieve tube is provided with a second diversion channel which can be connected with the production channel. Fluid is injected to make the second diversion channel separated from the production channel. The system can perform well testing on an upper layer and a lower layer.