The invention discloses a heeling in method of sugarcane seedlings. The method comprises the steps of putting macadimia nut bran, peat, vermiculite and river sand into soil holes of a heeling in plug, cutting part of leaves of sugarcane seedlings, and then transplanting the sugarcane seedlings into the soil holes to perform acclimatization before heeling in management period and field setting, wherein field setting can be performed after totally 20 to 25 days of heeling in and acclimatization. The method can improve the immunity and the disease resistance of the sugarcane seedlings, so as to obtain healthy and excellent sugarcane seedlings. The method is high in heeling in survival rate, simple to operate, resource-saving and low in cost.