The invention discloses a making method of nutritious rhizoma dioscoreae crisp chips. The method includes: preprocessing rhizoma dioscoreae, slicing, blanching, hardening, fixing, precooling, freeze drying and vacuum microwave processing. The method has the advantages that high-quality rhizoma dioscoreae is selected as the raw material; moisture in the rhizoma dioscoreae is replaced and solid contents are allowed to enter the rhizoma dioscoreae through blanching, hardening and fixing, and the crisp chips can keep certain shape and hardness; honey and inulin are added in fixing liquid to allow the crisp chips to be smooth in surface and high in crisp degree, and the nutrient contents of the crisp chips are increased; the replacement of the solid contents and nutrient contents and the moisture in the rhizoma dioscoreae is accelerated through microwave processing during fixing; high dewatering rate and crisp degree are achieved through staged variable-temperature freeze drying; the vacuum microwave processing is performed after the freeze drying, moisture is further removed, and the taste and crisp degree of the crisp chips are increased. The rhizoma dioscoreae crisp chips made by the method are bright in color, crisp in taste and rich in nutrition and have the fragrance of blueberry and the special flavor of the rhizoma dioscoreae.