The invention discloses an anastomosis instrument for a surgical operation. The anastomosis instrument comprises a shell, wherein a partition board is arranged in the middle of the right side of the shell, and an anastomosis nail channel is formed in the left side of the shell; a groove is formed in the middle of the upper side of the shell; a first spring is arranged on the upper part of the partition board, and the upper part of the first spring is connected to a movable handle; an anti-slipping sleeve is arranged in the middle of the movable handle, and the left end of the movable handle is fixedly connected to an anastomosis contact; a locating plate is arranged at the lower end of the shell on the right side of the anastomosis nail channel, and an anastomosis nail hook is arranged in the middle of the right side of the locating plate; an anastomosis nail mounting box is arranged on the lower part of the partition board, and the right side of the front end of the shell on the lower part of the partition board is connected to a box door by virtue of a hinge; a second spring is arranged on the inner wall of the right side of the anastomosis nail mounting box; a push plate is arranged at the left end of the second spring; and anastomosis nails are arranged in the anastomosis nail mounting box. By virtue of the instrument, the labor intensity of surgical doctors is reduced and wound suturing precision is enhanced.