The invention relates to a veterinary medicine, in particular to a medicine composition for preventing and treating the laying hen fatty liver syndrome and a preparation method thereof. The medicine composition is prepared from traditional Chinese medicine components and western medicine components according to the weight ratio of 2:1. The traditional Chinese medicine components include, by weight, 25-35 parts of Radix Salviae Miltiorrhizae, 18-22 parts of Schisandrachinensls (Turcz.) Baill, 8-12 parts of radix angelica sinensis, 8-12 parts of radix astragali, 8-12 parts of radix bupleuri, 8-12 parts of radix isatidis and 8-12 parts of liquorice roots. The western medicine components include, by weight, 80-120 parts of choline chloride, 100-150 parts of methionine, 1.8-2.2 parts of vitamin E, 0.001-0.0015 part of vitamin B, 80-120 parts of inositol and 150-200 parts of glucose. Western medicine compounds and traditional Chinese medicine such as Radix Salviae Miltiorrhizae are used in cooperation, and the treatment effect is improved.