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发明公开
CN105567680A 拟茎点霉RPB2基因扩增引物及其设计方法和应用 无效 - 撤回

拟茎点霉RPB2基因扩增引物及其设计方法和应用
摘要：
本发明公开了拟茎点霉RPB2基因扩增引物及其设计方法和应用。本发明设计的是针对拟茎点霉属真菌的通用引物。其上游引物PR2-F有44个碱基：CGCCAGGGTTTTCCCAGTCACGACATGGCCTACATGAAGCGATG，下游引物PR2-R有46个碱基：AGCGGATAACAATTTCACACAGGAATCTCACAATGCGTGTACATGT。本发明设计的RPB2引物对拟茎点霉属通用性强，扩增测序成功率高，具有合适的片段长度，便于高通量测序与分析，为拟茎点霉分子生态学研究以及检疫鉴定工作提供了重要工具。
摘要（英）：

The invention discloses a phomopsis RPB2 gene amplification primer and a design method and application thereof. The phomopsis RPB2 gene amplification primer is a universal primer aiming at phomopsis fungi. The upstream primer PR2-F of the phomopsis RPB2 gene amplification primer has 44 bases, to be more specific, CGCCAGGGTTTTCCCAGTCACGACATGGCCTACATGAAGCGATG, and the downstream primer PR2-R of the phomopsis RPB2 gene amplification primer has 46 bases, to be more specific, AGCGGATAACAATTTCACACAGGAATCTCACAATGCGTGTACATGT. The phomopsis RPB2 gene amplification primer has the advantages that the primer is high in universality to phomopsis, high in amplification sequencing success rate, appropriate in fragment length and convenient in high-throughput sequencing and analysis, and an important tool is provided for the molecular ecology researches and quarantine and identification of the phomopsis.

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