The invention discloses an enzymolysis method for a cellulose-contained material. The method includes the step that the cellulose-contained material is subjected to enzymolysis to obtain an enzymolysis product, wherein before performing enzymolysis on the cellulose-contained material, a premixing step is also included. In the premixing step, under stirring and extruding conditions, the cellulose-contained material is mixed with a pH value modifier first to regulate the pH value of the cellulose-contained material to be 4-6, the cellulose-contained material with the pH value of 4-6 is mixed with an enzyme, and the dry matter content of the cellulose-contained material is 15-50% by weight. According to the method, the premixing step before the enzymolysis step is added, the pH value of the cellulose-contained material is regulated first, and then the cellulose-contained material is premixed with a part of the enzyme; in this way, the cellulose-contained material is under conditions for full enzymolysis before enzymolysis, that is, the pH value of the cellulose-contained material is more uniform, sufficient premixing between the cellulose-contained material and a part of the enzyme is realized, the utilization rate of the enzyme is effectively increased, and the enzyme mixing efficiency is enhanced.