The invention belongs to the technical field of manufacturing of seamless tubes and discloses a preparation method for high-accuracy and high-strength titanium alloy seamless tubes. The process route of forging, forged bar drilling and cold rolling is adopted, high-strength titanium alloy bars are forged on a high-speed forging machine, and the comprehensive quality of the bars is improved; through holes are machined in the bars on a high-accuracy deep hole drilling machine, the dimension accuracy of tube blanks can be guaranteed, and the wall thickness deviation of the tube blanks is smaller than or equal to +/-1% of the normal wall thickness; the tube blanks undergo further rolling and annealing treatment, the needed boundary dimension and the tubes high in surface quality can be provided, and the structure property can be improved; a vertical annealing furnace is adopted for hanging heat treatment, and tube deformation in the heat treatment process is effectively avoided. According to the method, the equipment investment is small, the production process is simple, the labor intensity of workers is greatly lowered, products are high in dimensional accuracy and small in wall thickness deviation, the high-accuracy and high-strength titanium alloy seamless tubes with a medium tube diameter, a certain length and a certain tube thickness can be produced, and the yield of the tubes can be obviously improved.