The invention discloses an in-structural-floor wire conduit pre-buried mold. The in-structural-floor wire conduit pre-buried mold comprises a tank and insertion plates. The tank comprises vertical plates and supporting rods. The supporting rods are arranged in the middles of the vertical plates. Slots are formed in the ends of the vertical plates. The insertion plates are arranged in the slots and provided with groove holes. Pre-buried wire conduits penetrate through the insertion holes. According to the in-structural-floor wire conduit pre-buried mold and a construction method, operation is convenient, secondary protection processing is avoided, and construction efficiency is improved; a structural formwork can not be damaged; the pre-buried mold can be recycled, construction cost is saved, and construction waste is reduced; the pre-buried mold is safe and environmentally friendly, building rubbish is avoided, and the building construction environment is improved.