The invention provides an ELISA kit for detecting the residual quantity of monensin in an animal-derived food, and relates to an immunoassay detection technology. The kit comprises an enzyme labeled plate coated with a monensin antigen, a monensin antibody, a monensin standard substance, a standard substance dilution solution, an enzyme labeled secondary antibody, a substrate coloring solution, a washing buffer solution and a stopping solution. A monensin monoclonal antibody is prepared in the invention. The kit adopts the highly specific monensin monoclonal antibody, and a main reagent is provided with as an operating fluid, so the operating steps are reduced, the time is saved, and the residual quantity of monensin in a meat product especially is detected.