The invention discloses a mink canine parvovirus enteritis pathogen antigen colloidal gold detection test strip and a production method thereof, and relates to the technical field of detection of fur animal viral infectious disease pathogens. The colloidal gold detection test strip comprises a sample pad, a colloidal gold pad, an NC film and an absorption pad, the detection line of the NC film is sprayed with a VP2 protein, and the quality control line of the NC film is sprayed with a goat-anti-mouse second antibody. The production method of the mink canine parvovirus enteritis pathogen antigen colloidal gold detection test strip mainly comprises the following steps: carrying out prokaryotic expression to obtain mink parvovirus (MEV) VP2 and VP1 proteins, and purifying the proteins; preparing an MEV monoclonal antibody; preparing a colloidal gold solution; preparing a colloidal gold antibody solution; preparing the colloidal gold pad; preprocessing the NC film; and assembling. The test strip is helpful for purifying the mink canine parvovirus enteritis in fur animal culturing farms, and is convenient for basic veterinary personnel to operate.