The invention relates to a layered cell coculturing device and method and belongs to the technical field of cell culturing. The layered cell coculturing device comprises a culture bottle (1) and a partition board (2) arranged in the culture bottle (1), and the partition board (2) is connected with the side walls of the culture bottle (1) and is parallel to a culture bottle bottom (4). By means of the culture bottle, coculture cells different in buoyancy can be achieved, the internal environments of the animal cells can be simulated, raw materials can be saved, internal and external exchange of gas is facilitated, liquid leakage is avoided, and cell pollution is effectively prevented.