The invention relates to the technical field of road construction, in particular to a multi-layer support inspection well. The multi-layer support inspection well comprises bottom-layer supports, middle-layer supports, a top-layer support, an upper well lid and auxiliary well lids. A first well lid supporting stand, second well lid supporting stands and third well lid supporting stands are disposed on the inner side wall of the top-layer support, the inner side walls of the middle-layer supports and the inner side walls of the bottom-layer supports correspondingly. The second well lid supporting stands or the third well lid supporting stands are used for building the auxiliary well lids. The upper well lid is fixed to the first well lid supporting stand of the top-layer support through anti-theft abnormal-shaped bolts. The outer side wall of the upper well lid and the upper end part of the inner side wall of the top-layer support are connected through clamping grooves and clamping nails, the lower end parts of the outer side walls of the middle-layer supports and the upper end parts of the inner side walls of the bottom-layer supports are connected through clamping grooves and clamping nails, and the lower end part of the outer side wall of the top-layer support and the upper end parts of the inner side walls of the middle-layer supports are connected through clamping grooves and clamping nails. Outwards extending bottom plates with through holes are disposed on the bottom-layer supports and fixing parts penetrate through the through holes to fix the outwards extending bottom plates into a road surface base layer. The multi-layer support inspection well has multiple guarantees of prevention of thievery, prevention of making noise, prevention of falling, prevention of displacement and the like.