The invention discloses a paddy rice seedling culture method of selenium-enriched rice. The paddy rice seedling culture method of selenium-enriched rice comprises the following steps including seedbed selection and treatment, rice seed selection and treatment, sowing, film covering, film uncovering treatment and pest prevention and curing. The seedling culture method has the advantages that the steps are complete; rice seeds are treated by a complex solution; the selenium content of the rice seeds is increased; in addition, the disease resistance and the insect resistance of the rice seeds are improved, so that the rice seeds can normally sprout and grow; through reasonable planting steps and treatment means, the quality of rice seedlings cultured by the seedling culture method is better, so that the quality and the yield of harvested selenium-enriched rice are guaranteed.