The invention relates to an Au-doped copper nano-cluster self-assembly fluorescent material, a preparing method and application thereof to LED packaging, and belongs to the technical field of LED packaging materials. The self-assembly fluorescent material is formed by copper nano-cluster through self-assembly, Au element is doped in the fluorescent material (the Au element accounts for 0.003-80% of the total metallic element molar ratio), and fluorescent powder with the fluorescent color adjustable continuously is obtained through doping. The preparing method of the self-assembly fluorescent material is simple and fast, the raw materials are low in cost, the technology is free of pollution, the fluorescent color is adjustable, the quantum yield is high, and stability is high; the fluorescent material is large in size, energy transfer is not prone to occurring after mixing, and the self-assembly fluorescent material can be applied to the field of preparing high-color-rendering white light LEDs.