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发明公开
CN105602991A 一种乙脑假病毒及其制备方法 失效 - 权利终止

一种乙脑假病毒及其制备方法
摘要：
本发明公开了一种重组载体，它是中国典型培养物保藏中心保藏的保藏号为CCTCC NO：M2015570的重组菌DH5α/pcDNA3.1-SPME携带的重组质粒pcDNA3.1-SPME。本发明还公开了一种制备乙脑假病毒的试剂盒及其用途。本发明还公开了一种乙脑假病毒的制备方法，一种重组菌。本发明构建的重组载体pcDNA3.1-SPME，可以高效的获得乙脑假病毒，获得的乙脑假病毒滴度高，具有良好的应用前景。
摘要（英）：

The invention discloses recombinant vectors. The recombinant vectors are recombinant plasmids pcDNA3.1-SPME carried by recombinant bacteria DH5alpha/pcDNA3.1-SPM, the collection number of the recombinant plasmids in the China center for type culture collection (CCTCC) is CCTCC NO:M2015570. The invention further discloses a kit for preparing epidemic encephalitis B pseudoviruses and an application thereof. The invention further discloses a preparing method of the pidemic encephalitis B pseudoviruses and the recombinant bacteria. By means of the constructed recombination vectors pcDNA3.1-SPME, the epidemic encephalitis B pseudoviruses can be efficiently obtained, the titer of the obtained pidemic encephalitis B pseudoviruses can be high, and good application prospects can be achieved.

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