The invention discloses a surface plasma resonance sensor chip comprising a culture cavity, a heating device, a culture solution inflow channel and a prism. The culture cavity is provided with a culture solution inflow port and a culture solution outflow port. The heating device comprises two layers of heat conductive metal and a high-resistivity membrane interlayer structure clamped between the two layers of heat conductive metal and is of a through structure, wherein the culture cavity is embedded in the through structure. The culture solution inflow channel makes a culture solution in the culture solution inflow channel flow through one surface of the heating device before entering the culture solution inflow port of the culture cavity. The prism is located on the lower portion of the culture cavity and closely connected with the culture cavity in a sealing mode, the surface, connected with the culture cavity, of the prism is plated with a precious metal membrane. The sensor chip also comprises a transparent heat-insulating shell, and the culture cavity can be layered. Thus, the temperature of a cell growing environment can be evenly and stably maintained for a long time, and cell response detection accuracy is improved.