The invention discloses a uric acid detection electrochemical sensor and preparation and application thereof.The electrochemical sensor comprises a glass carbon electrode and a film formed by coating the surface of the glass carbon electrode with electrically reduced graphene oxide and cuprous oxide nano particles.According to the main preparation process of the electrochemical sensor, graphite powder is used as a raw material, graphite oxide is prepared through an improved Hummers method and ultrasonically dispersed in distilled water to obtain graphene oxide sol, then the cuprous oxide nano particles are immobilized on a graphene oxide sheet by ultrasonic action, the surface of the glass carbon electrode is modified with a graphene oxide-cuprous oxide nanocomposite through a dispensing method, and finally the graphene oxide is reduced by a constant potential to obtain the electrically reduced graphene oxide and cuprous oxide nano-composite film.The uric acid detection electrochemical sensor has the advantages of being high in sensitivity, wide in linear range, low in quantity requirement, high in anti-jamming capability, short in detection time, low in cost and the like.