The invention discloses a quantum-dot immunofluorescence kit for simultaneously detecting gentamycin and quinolone medicines, and at the same time, the invention also discloses a preparation method of ciprofloxacin hapten, corresponding artificial antigen and a quantum dot-labelled monoclonal antibody. The kit takes a conjugate of gentamycin semiantigen and carrier protein as well as the conjugate of the ciprofloxacin semiantigen and carrier protein as a primordial covering, and takes a mixture of a quantum dot-labelled gentamycin specific antibody and a ciprofloxacin specific antibody as a detection antibody. The preparation method is simple and feasible, has the advantages of low cost and high semiantigen yield, and the quantum-dot immunofluorescence kit has the advantages of simpleness, rapidity, large sample processing amount, high sensitivity, and strong specificity. The quantum-dot immunofluorescence kit can be used for self-control of quality and food safety supervision of a food production enterprise, and routine screening of a detection mechanism, and can be used as an auxiliary means for animal pharmacological and toxicologic researches in scientific research.