A display device, a glasses-free three-dimensional display system and virtual reality (VR) glasses are disclosed. The display device includes a backlight; a polarizer disposed on a light-emitting side of the backlight and a plurality of liquid crystal displays (LCDs) stacked on a light-emitting side of the polarizer. Each LCD includes a plurality of light adjusting units; the light adjusting units of the plurality of LCDs are staggered from each other; and the light adjusting unit is configured to allow light emitted by the backlight to be refracted and emitted to a target position or be totally reflected.